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uterine horn  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc uterine horn
    Uterine Horn, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10024 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/uterine+horn/RIPA+Buffer/pmc11441723-106-14-27
    Average 99 stars, based on 10024 article reviews
    uterine horn - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus
    Article Snippet: The number of ChAT-immunoreactive cells was manually counted in representative glands and luminal epithelium in a subset of animals ( n = 6) to determine the percentage of ChAT-positive cells from total cells (estimated by nuclei count).in representative glands and luminal epithelium in a subset of animals ( n = 6) to determine the percentage of ChAT-positive cells from total cells (estimated by nuclei count). ... Tissue lysate preparation and protein quantification Uterine samples from the cranial region of the uterine horn were minced and homogenized in RIPA buffer (1:10 w/v; Cat. 9806; Cell Signaling, Danvers, MA; 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na 3 VO 4 , 1 μg/mL leupeptin) supplemented with protease inhibitors (Cat. A32953; Pierce, Rockford, IL; AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A).. Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.

    Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus
    Article Snippet: Uterine samples from the cranial region of the uterine horn were minced and homogenized in RIPA buffer (1:10 w/v; Cat. 9806; Cell Signaling, Danvers, MA; 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na 3 VO 4 , 1 μg/mL leupeptin) supplemented with protease inhibitors (Cat. A32953; Pierce, Rockford, IL; AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A).. Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus
    Article Snippet: The number of ChAT-immunoreactive cells was manually counted in representative glands and luminal epithelium in a subset of animals ( n = 6) to determine the percentage of ChAT-positive cells from total cells (estimated by nuclei count).in representative glands and luminal epithelium in a subset of animals ( n = 6) to determine the percentage of ChAT-positive cells from total cells (estimated by nuclei count). ... Tissue lysate preparation and protein quantification Uterine samples from the cranial region of the uterine horn were minced and homogenized in RIPA buffer (1:10 w/v; Cat. 9806; Cell Signaling, Danvers, MA; 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na 3 VO 4 , 1 μg/mL leupeptin) supplemented with protease inhibitors (Cat. A32953; Pierce, Rockford, IL; AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A).. Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.

    Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus
    Article Snippet: Uterine samples from the cranial region of the uterine horn were minced and homogenized in RIPA buffer (1:10 w/v; Cat. 9806; Cell Signaling, Danvers, MA; 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na 3 VO 4 , 1 μg/mL leupeptin) supplemented with protease inhibitors (Cat. A32953; Pierce, Rockford, IL; AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A).. Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.

    Lysis:

    Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus
    Article Snippet: The number of ChAT-immunoreactive cells was manually counted in representative glands and luminal epithelium in a subset of animals ( n = 6) to determine the percentage of ChAT-positive cells from total cells (estimated by nuclei count).in representative glands and luminal epithelium in a subset of animals ( n = 6) to determine the percentage of ChAT-positive cells from total cells (estimated by nuclei count). ... Tissue lysate preparation and protein quantification Uterine samples from the cranial region of the uterine horn were minced and homogenized in RIPA buffer (1:10 w/v; Cat. 9806; Cell Signaling, Danvers, MA; 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na 3 VO 4 , 1 μg/mL leupeptin) supplemented with protease inhibitors (Cat. A32953; Pierce, Rockford, IL; AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A).. Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.

    Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus
    Article Snippet: Uterine samples from the cranial region of the uterine horn were minced and homogenized in RIPA buffer (1:10 w/v; Cat. 9806; Cell Signaling, Danvers, MA; 20 mM Tris–HCl, 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na 3 VO 4 , 1 μg/mL leupeptin) supplemented with protease inhibitors (Cat. A32953; Pierce, Rockford, IL; AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A).. Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.Lysates were incubated on ice for 5 min and then centrifuged at 14,000 g for 10 min at 4 °C.



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